The EF65-1000 made its debut in 1969 and pulled various cars ranging from blue (sleeper) trains to freight cars in DC electrified zones on plains throughout Japan. Different from the EF65-0 and EF65-500, this version has a through door on its front to facilitate double heading. The quipment is also cold weather reinforced and insulated against winter use in snowy Tohoku and Jo-etsu Mainlines. Of the 139 cars produced in total, all are different among themselves, depending on when they were produced and any special paint specifications they may have received.

Actual locomotive different paint scheme from shown.